Biology

Cell organelles and their functions

Organelles are the small working parts inside a cell, each with its own job. The nucleus stores DNA, ribosomes build proteins, the endoplasmic reticulum and Golgi apparatus finish and ship them, and mitochondria release energy as ATP. Plant cells also have chloroplasts, a cell wall and a large central vacuole. Bacteria are prokaryotes: they have ribosomes but no nucleus and no membrane-bound organelles.

Updated

Key ideas

There are two kinds of cells. Prokaryotic cells (bacteria and archaea) are small and simple: their DNA sits in a region called the nucleoid, with no membrane around it. Eukaryotic cells (plants, animals, fungi, protists) keep their DNA inside a nucleus and have many organelles wrapped in membranes.

Organelles: what they do and where you find them (OpenStax Biology 2e, 4.2 to 4.4)
PartJobAnimalPlantBacteria
Plasma membraneControls what enters and leavesYesYesYes
CytoplasmGel-like fluid where many reactions happenYesYesYes
RibosomesBuild proteinsYesYesYes
NucleusHolds DNA and controls the cellYesYesNo
Rough ERStudded with ribosomes, makes and folds proteinsYesYesNo
Smooth ERMakes lipids, breaks down some toxins, stores calciumYesYesNo
Golgi apparatusSorts, tags and packages proteins and lipidsYesYesNo
MitochondriaCellular respiration: make most of the cell's ATPYesYesNo
ChloroplastsPhotosynthesisNoYesNo
LysosomesDigest worn-out parts and food with enzymesYesNo (the vacuole does this job)No
Large central vacuoleStores water, keeps the cell firmNoYesNo
Cell wallRigid outer layer for supportNoYes (cellulose)Yes (most; peptidoglycan)
CentrosomeOrganizes microtubules for cell divisionYesNoNo

Worked examples

Example 1: follow a protein out of the cell

Problem A pancreas cell makes insulin, a protein that leaves the cell. List the parts it passes through, in order.

  1. The instructions start as DNA in the nucleus, which is copied into mRNA.
  2. The mRNA goes to a ribosome on the rough ER, which builds the protein into the ER.
  3. A small bubble called a vesicle carries it to the Golgi apparatus, which modifies and packages it.
  4. Another vesicle carries it to the plasma membrane and releases it outside the cell.

Answer Nucleus, rough ER ribosome, Golgi apparatus, plasma membrane

Example 2: identify the cell from its parts

Problem Under a microscope, a cell has a cell wall, ribosomes and a plasma membrane, but no nucleus and no chloroplasts. What kind of cell is it?

  1. No nucleus means it cannot be a plant or animal cell. It is a prokaryote.
  2. A cell wall and ribosomes fit bacteria (see the table).

Answer A prokaryotic cell, such as a bacterium

Example 3: which cell needs more mitochondria?

Problem A heart muscle cell and a fat storage cell are the same size. Which would you expect to have more mitochondria, and why?

  1. Mitochondria make ATP, the cell's energy currency.
  2. Heart muscle contracts every second of your life, so it uses a lot of ATP. A fat cell mostly stores energy.

Answer The heart muscle cell, because it uses far more ATP

Common mistakes and how to fix them

  • Saying plants do not have mitochondria. They do. Plants make sugar in chloroplasts and then break it down for ATP in mitochondria, just like animals.
  • Saying bacteria have no ribosomes. Every cell needs proteins, so every cell has ribosomes. Bacteria lack a nucleus and membrane-bound organelles.
  • Mixing up cell wall and cell membrane. Every cell has a membrane. Plants, fungi and most bacteria also have a wall outside it.
  • Calling the nucleus the brain. It is closer to a library of instructions. The DNA is read when proteins are needed.

Practice questions

  1. Which organelle makes most of a cell's ATP?

    1. Ribosome
    2. Mitochondrion
    3. Golgi apparatus
    4. Nucleus
    Show answer

    Answer: Mitochondrion

    Mitochondria carry out the later stages of cellular respiration, where most ATP is made.

  2. Which part is found in plant cells but not in animal cells?

    1. Mitochondria
    2. Ribosomes
    3. Chloroplasts
    4. Plasma membrane
    Show answer

    Answer: Chloroplasts

    Chloroplasts carry out photosynthesis. Both plant and animal cells have mitochondria, ribosomes and a plasma membrane.

  3. Which part do bacteria have?

    1. Nucleus
    2. Ribosomes
    3. Mitochondria
    4. Golgi apparatus
    Show answer

    Answer: Ribosomes

    Bacteria are prokaryotes with no membrane-bound organelles, but they still need ribosomes to build proteins.

  4. Which organelle sorts, tags and packages proteins for shipping?

    1. Smooth ER
    2. Lysosome
    3. Golgi apparatus
    4. Vacuole
    Show answer

    Answer: Golgi apparatus

    Proteins from the rough ER travel to the Golgi apparatus, which modifies them and sends them on in vesicles.

  5. A cell has a nucleus, mitochondria, a cell wall, chloroplasts and a large central vacuole. What is it?

    1. Animal cell
    2. Plant cell
    3. Bacterium
    Show answer

    Answer: Plant cell

    A nucleus rules out bacteria. A cell wall, chloroplasts and a large central vacuole together point to a plant cell.

Frequently asked questions

Why do mitochondria and chloroplasts have their own DNA?

The endosymbiotic theory says both came from bacteria that were taken in by an early cell and lived on inside it. They still have their own circular DNA and ribosomes, and they divide on their own, much like bacteria. OpenStax covers the evidence in its section on eukaryotic origins.

What is the difference between rough and smooth ER?

Rough ER has ribosomes on its surface, so it looks bumpy and makes proteins, especially ones that will be shipped out or put in membranes. Smooth ER has no ribosomes and makes lipids, breaks down some toxins, and stores calcium in muscle cells.

Do I need to know every organelle for a test?

Most high school tests focus on the nucleus, ribosomes, ER, Golgi, mitochondria, chloroplasts, vacuoles, lysosomes, cell membrane and cell wall, and on comparing plant, animal and bacterial cells. AP Biology adds how the endomembrane system works together and why cells are small.

Sources

  1. OpenStax Biology 2e, 4.2 Prokaryotic Cells, accessed October 1, 2026
  2. OpenStax Biology 2e, 4.3 Eukaryotic Cells, accessed October 1, 2026
  3. OpenStax Biology 2e, 4.4 The Endomembrane System and Proteins, accessed October 1, 2026

Try asking Ducky

  • “Look at my labeled cell diagram. Did I mix up any organelles?”
  • “Quiz me on plant versus animal cells until I get five right in a row.”
  • “Explain the endomembrane system with a picture I can follow.”

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